{"product_id":"9780123847447","title":"Contemporary Enzyme Kinetics and Mechanism: Reliable Lab Solutions","description":"Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents.\u003cbr\u003e\u003cb\u003eContemporary Enzyme Kinetics and Mechanism, Second Edition\u003c\/b\u003e presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of \u003cb\u003eMethods in Enzymology\u003c\/b\u003e. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics.\u003cbr\u003eThe book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.\u003cbr\u003eOf the seventeen chapters presented in this new edition, ten did not previously appear in the first edition.\u003cul\u003e\n\u003cli\u003eTransient kinetic approaches to enzyme mechanisms\u003c\/li\u003e\n\u003cli\u003eDesigning initial rate enzyme assay\u003c\/li\u003e\n\u003cli\u003eDeriving initial velocity and isotope exchange rate equations\u003c\/li\u003e\n\u003cli\u003ePlotting and statistical methods for analyzing rate data\u003c\/li\u003e\n\u003cli\u003eCooperativity in enzyme function\u003c\/li\u003e\n\u003cli\u003eReversible enzyme inhibitors as mechanistic probes\u003c\/li\u003e\n\u003cli\u003eTransition-state and multisubstrate inhibitors\u003c\/li\u003e\n\u003cli\u003eAffinity labeling to probe enzyme structure and function\u003c\/li\u003e\n\u003cli\u003eMechanism-based enzyme inactivators\u003c\/li\u003e\n\u003cli\u003eIsotope exchange methods for elucidating enzymatic catalysis\u003c\/li\u003e\n\u003cli\u003eKinetic isotope effects in enzyme catalysis\u003c\/li\u003e\n\u003cli\u003eSite-directed mutagenesis in studies of enzyme catalysis\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Elsevier Science","offers":[{"title":"Default Title","offer_id":47153761812720,"sku":"9780123847447","price":81.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0737\/7593\/9824\/files\/9780123847447_p0.jpg?v=1763639917","url":"https:\/\/shop-qa.barnesandnoble.com\/products\/9780123847447","provider":"Barnes \u0026 Noble (DEV)","version":"1.0","type":"link"}