{"product_id":"9781118829448","title":"RNA Interference (RNAi)","description":"\u003cp\u003eThe 2006 Nobel Prize in Physiology or Medicine was awarded to Andrew Z. Fire and Craig C. Mello for their discovery of RNA interference (RNAi). RNAi is a biological process in which RNA molecules can silence (inhibit) or up- or down-regulate gene expression, typically by causing the destruction of specific messenger RNA (mRNA) molecules. This collection of articles begins with an introduction to RNAi, and proceeds to describe uses of this technology in various approaches to disease treatment, including gene therapy. Several laboratory protocols for silencing genes via RNAi are also provided, as are protocols for down-regulating and then rescuing those down-regulated genes, which demonstrates specificity of the approach.\u003c\/p\u003e \u003cp\u003eThis e-book — a curated collection from eLS, WIREs, and Current Protocols — offers a fantastic introduction to the field of RNA interference for students or interdisciplinary collaborators.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eTable of Contents:\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIntroduction\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview of Gene Silencing by RNA Interference\u003cbr\u003e \u003ci\u003eCurrent Protocols in Nucleic Acid Chemistry\u003c\/i\u003e\u003cbr\u003e Daniel H. Kim, John J. Rossi\u003c\/p\u003e \u003cp\u003eBiogenesis and function of endogenous and exogenous siRNAs\u003cbr\u003e \u003ci\u003eWIREs RNA\u003c\/i\u003e\u003cbr\u003e Nicholas M. Snead, John J. Rossi\u003c\/p\u003e \u003cp\u003e\u003cb\u003eTechniques\u0026amp;Applications\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eSmall Silencing RNAs and Gene Therapy\u003cbr\u003e \u003ci\u003eeLS\u003c\/i\u003e\u003cbr\u003e Dirk Grimm\u003c\/p\u003e \u003cp\u003eRNAi Screening: New Approaches, Understandings, and Organisms\u003cbr\u003e \u003ci\u003eWIREs RNA\u003c\/i\u003e\u003cbr\u003e Stephanie E. Mohr, Norbert Perrimon\u003c\/p\u003e \u003cp\u003eDelivery of RNAi mediators\u003cbr\u003e \u003ci\u003eWIREs RNA\u003c\/i\u003e\u003cbr\u003e Lance P. Ford, Masoud M. Toloue\u003c\/p\u003e \u003cp\u003e\u003cb\u003eProtocols\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eSilencing of Gene Expression in Cultured Cells Using Small Interfering RNAs\u003cbr\u003e \u003ci\u003eCurrent Protocols in Cell Biology\u003c\/i\u003e\u003cbr\u003e Kumi Sakurai, Pritsana Chomchan, John J. Rossi\u003c\/p\u003e \u003cp\u003eGene Down-Regulation with Short Hairpin RNAs and Validation of Specificity by Inducible Rescue in Mammalian Cells\u003cbr\u003e \u003ci\u003eCurrent Protocols in Cell Biology\u003c\/i\u003e\u003cbr\u003e Hoi Tang Ma, Randy Y.C. Poon\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47130288128240,"sku":"9781118829448","price":34.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0737\/7593\/9824\/files\/9781118829448_p0.jpg?v=1763695632","url":"https:\/\/shop-qa.barnesandnoble.com\/products\/9781118829448","provider":"Barnes \u0026 Noble (DEV)","version":"1.0","type":"link"}