{"product_id":"9781493938087","title":"Skeletal Muscle Regeneration in the Mouse: Methods and Protocols","description":"This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility;\u003cbr\u003eforce measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format,\u003cbr\u003echapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cp\u003eCutting edge and practical, \u003ci\u003eSkeletal Muscle Regeneration in the Mouse: Methods and Protocols \u003c\/i\u003eis an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system.\u003c\/p\u003e","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":47033166070000,"sku":"9781493938087","price":139.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0737\/7593\/9824\/files\/9781493938087_p0.jpg?v=1763665834","url":"https:\/\/shop-qa.barnesandnoble.com\/products\/9781493938087","provider":"Barnes \u0026 Noble (DEV)","version":"1.0","type":"link"}