{"product_id":"9781493966899","title":"Metagenomics: Methods and Protocols","description":"\u003cp\u003eThis second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E\u003ci\u003e.\u003c\/i\u003ecoli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. \u003ci\u003eMetagenomics: Methods and Protocols, Second Edition\u003c\/i\u003e also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites. Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003eComprehensive and cutting-edge, \u003ci\u003eMetagenomics: Methods and Protocols, Second Edition\u003c\/i\u003e is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":47052275319024,"sku":"9781493966899","price":119.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0737\/7593\/9824\/files\/9781493966899_p0.jpg?v=1763664977","url":"https:\/\/shop-qa.barnesandnoble.com\/products\/9781493966899","provider":"Barnes \u0026 Noble (DEV)","version":"1.0","type":"link"}