{"product_id":"9781904842453","title":"Expression Systems","description":"\u003cp\u003eProtein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.\u003cbr\u003e \u003ci\u003eExpression Systems: Methods Express\u003cb\u003e \u003c\/b\u003e\u003c\/i\u003euniquely fills this need. It covers expression across a broad range of systems, including the following:\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cul\u003e \u003cli\u003eBaculovirus expression vectors\u003c\/li\u003e \u003cli\u003eCHO cells\u003c\/li\u003e \u003cli\u003e\u003ci\u003eE. coli\u003c\/i\u003e\u003c\/li\u003e \u003cli\u003eHEK293-EBNA1 cells\u003c\/li\u003e \u003cli\u003e\n\u003ci\u003eLactococcus lactis \u003c\/i\u003eand other gram positive bacteria\u003c\/li\u003e \u003cli\u003e\u003ci\u003eS. cerevisiae\u003c\/i\u003e\u003c\/li\u003e \u003cli\u003eTransfected insect cells\u003c\/li\u003e \u003cli\u003e\u003ci\u003ePichia pastoris\u003c\/i\u003e\u003c\/li\u003e \u003cli\u003eMammalian cells using BacMam viruses\u003c\/li\u003e \u003cli\u003eLentiviral vectors\u003c\/li\u003e \u003cli\u003eWheat germ cell-free system\u003c\/li\u003e \u003c\/ul\u003e\u003cp\u003eThe book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.\u003cbr\u003e Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.\u003cbr\u003e In summary, \u003ci\u003eExpression Systems: Methods Express \u003c\/i\u003eis a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.\u003c\/p\u003e","brand":"Scion Publishing Ltd.","offers":[{"title":"Default Title","offer_id":47047438008560,"sku":"9781904842453","price":125.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0737\/7593\/9824\/files\/9781904842453_p0.jpg?v=1763614096","url":"https:\/\/shop-qa.barnesandnoble.com\/products\/9781904842453","provider":"Barnes \u0026 Noble (DEV)","version":"1.0","type":"link"}