{"product_id":"9783836495554","title":"DNA Replicons - Next-Generation Vaccines Against Allergy","description":"In this study, DNA vaccines based on alphaviral replicases (DNA replicons) were evaluated with respect to their protective and therapeutic capacity for treatment of type I allergy. DNA replicons display specific danger signals and offer an excellent safety profile based on apoptotic elimination of transfected host cells thereby preventing undesirable long-term expression of the transfected gene. Using the model allergen beta-Galactosidase in a protective approach, the results showed that a replicase-based DNA vaccine can induce a comparable immune response at a more than 1000-fold lower dose than a conventional DNA vaccine. DNA replicon doses as low as 10 ng significantly prevented from IgE induction by subcutaneous protein sensitization. Furthermore, in a therapeutic approach with the same construct, DNA replicons balanced an ongoing allergic response. These data were confirmed with a DNA replicon encoding the clinically relevant timothy grass pollen allergen Phl p 5. In addition, lung pathology could be prevented by pre-vaccination with DNA replicons as measured by the magnitude of eosinophilia, goblet cell hyperplasia, IL-5 production and proliferation of endothelial cells.","brand":"VDM Verlag Dr. Mueller E.K.","offers":[{"title":"Default Title","offer_id":47042213871856,"sku":"9783836495554","price":76.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0737\/7593\/9824\/files\/9783836495554_p0.jpg?v=1763688446","url":"https:\/\/shop-qa.barnesandnoble.com\/products\/9783836495554","provider":"Barnes \u0026 Noble (DEV)","version":"1.0","type":"link"}