{"product_id":"9789814579568","title":"Synthetic Biology: A Lab Manual: A Lab Manual","description":"\u003cp\u003e\u003ci\u003eSynthetic Biology: A Lab Manual\u003c\/i\u003e is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAn inspiring foreword is written by another pioneer in the field, Harvard's George Church:\u003c\/b\u003e\u003cbr\u003e\u003ci\u003e“Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”\u003c\/i\u003e\u003c\/p\u003e\u003cb\u003eContents:\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eIntroduction:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003eWhat is Synthetic Biology, Exactly?\u003c\/li\u003e\n\u003cli\u003eThe iGEM Outbreak\u003c\/li\u003e\n\u003cli\u003eA Synthetic Biology Lab Manual\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eGenes, Chromoproteins and Antisense RNAs:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003e\n\u003ci\u003eE. coli\u003c\/i\u003e DNA: Chromosomes, Plasmids and Copy Number\u003c\/li\u003e\n\u003cli\u003eCoupling of Transcription and Translation in Bacteria\u003c\/li\u003e\n\u003cli\u003ePromoter and Terminator for Transcription\u003c\/li\u003e\n\u003cli\u003eRibosome Binding Site (RBS)\u003c\/li\u003e\n\u003cli\u003eCodon Bias\u003c\/li\u003e\n\u003cli\u003eChromoproteins\u003c\/li\u003e\n\u003cli\u003eSmall Regulatory RNAs (sRNAs)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eLab Rooms and Equipment:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003eThe Physical Lab Spaces\u003c\/li\u003e\n\u003cli\u003eEquipment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eSafety is Priority #1:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003eFires\u003c\/li\u003e\n\u003cli\u003eChemicals\u003c\/li\u003e\n\u003cli\u003eBiological Safety and Disposal\u003c\/li\u003e\n\u003cli\u003eDangerous Equipment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eLab Course Projects:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003eTime and Resources\u003c\/li\u003e\n\u003cli\u003eProject Overview and Learning Objectives\u003c\/li\u003e\n\u003cli\u003eThe Lab Notebook\u003c\/li\u003e\n\u003cli\u003eLab Section 1. Preparation of Chemical Solutions and Agar Plates\u003c\/li\u003e\n\u003cli\u003eLab Section 2. Coloring Bacteria by Adding a Promoter to a Chromoprotein Gene\u003c\/li\u003e\n\u003cli\u003eLab Section 3: Rational Engineering of Chromoprotein Expression Level\u003c\/li\u003e\n\u003cli\u003eLab Section 4. Other Experiments\u003c\/li\u003e\n\u003cli\u003eThe “Dreaded” Exam\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eProtocols:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003eIntroduction\u003c\/li\u003e\n\u003cli\u003eProtocol 1. Preparation of Solutions and Agar Plates\u003c\/li\u003e\n\u003cli\u003eProtocol 2. Overnight Cultures with Antibiotics, and Glycerol Stocks\u003c\/li\u003e\n\u003cli\u003eProtocol 3. BioBrick™ 3A Assembly and Gel Analysis\u003c\/li\u003e\n\u003cli\u003eProtocol 4. Agarose Gel Electrophoresis\u003c\/li\u003e\n\u003cli\u003eProtocol 5. Preparation of Competent \u003ci\u003eE. coli\u003c\/i\u003e Cells Using CaCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/li\u003e\n\u003cli\u003eProtocol 6. Transformation of CaCl\u003csub\u003e2\u003c\/sub\u003e-Competent \u003ci\u003eE. coli\u003c\/i\u003e Cells\u003c\/li\u003e\n\u003cli\u003eProtocol 7. Bacterial Re-Streaking Techniques\u003c\/li\u003e\n\u003cli\u003eProtocol 8. Lysis of \u003ci\u003eE. coli\u003c\/i\u003e Cells with Lysozyme\u003c\/li\u003e\n\u003cli\u003eProtocol 9. Polymerase Chain Reaction (PCR)\u003c\/li\u003e\n\u003cli\u003eProtocol 10. Inverse PCR Mutagenesis\u003c\/li\u003e\n\u003cli\u003eProtocol 11. Colony PCR\u003c\/li\u003e\n\u003cli\u003eProtocol 12. Gibson Assembly\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eAdvanced Methods:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003eFlow Cytometry and Cell Sorting\u003c\/li\u003e\n\u003cli\u003eRecombination in Plasmids and the Chromosome\u003c\/li\u003e\n\u003cli\u003eElectrocompetent Cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003eThe International Genetically Engineered Machine (iGEM) Competition:\u003c\/i\u003e\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003eHow to Start an iGEM Team\u003c\/li\u003e\n\u003cli\u003eUppsala iGEM 2011 — Show Color with Color\u003c\/li\u003e\n\u003cli\u003eUppsala iGEM 2012 — Resistance is Futile\u003c\/li\u003e\n\u003cli\u003eUppsala iGEM 2013 — Lactonutritious — It's Delicious\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cb\u003e\u003ci\u003eAppendices\u003c\/i\u003e\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cbr\u003e\u003cb\u003eReadership:\u003c\/b\u003e Students and researchers in biotechnology, cell\/molecular biology and genetics.\u003cbr\u003e","brand":"World Scientific Publishing Company, Incorporated","offers":[{"title":"Default Title","offer_id":47143740113136,"sku":"9789814579568","price":30.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0737\/7593\/9824\/files\/9789814579568_p0.jpg?v=1763691687","url":"https:\/\/shop-qa.barnesandnoble.com\/products\/9789814579568","provider":"Barnes \u0026 Noble (DEV)","version":"1.0","type":"link"}