Genomic DNA Isolation and Purification of Two Endemic Medicinal Plants (Pterocarpus Santalinus Linn.F & Pimpinella Tirupatiensis Bal&Subr) of Seshachalam Hills, Tirumala.

Ptercarpus santalinus is an important endemic medicinal plant with high medicinal values
that interfere with DNA extraction procedures and qualitative, quantitative agaros gel
electrophoresis. An effective and low-cost protocol for isolating genomic DNA from the roots
of Pterocarpus santilinus and Pimpinella tirupatiensis was described in this paper. It involved
a modified CTAB method with distilled water pretreating samples. The A260/A280
absorbance ratio of extracted DNA was found to be free from polysaccharide, poly-phenols
and tannins contaminants ranged from 2.2 to 2.8 within the high level of purity.