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Gormley Publishing
Methods of Chromatography
Methods of Chromatography
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The Neuhaus Science Projects Guide, Methods of Chromatography, is written for students in the junior/senior high school. This booklet describes some of the basic techniques used to separate and identify chemicals. There are excellent photographs and illustrations throughout.
Methods and topics discussed:
Paper Chromatography:-- A Simple Experiment Tells All • Chromatography • How the Method of Paper Chromatography Works • Solvents • The Spot Technique of Paper Chromatography • Materials Needed • Matching Controls • Reagents • Preparation of Unidentified Mixtures Prior to Separation • Technique of Paper Chromatography • Practice Runs with Food Dyes • Testing Unknowns • Some Hazardous Solvents to Avoid • Selecting a Solvent • Some Visualization Reagents • Quantitative Analysis.
Thin Layer Chromatography (TLC):-- How to Coat Glass Microscope Slides • TLC Kits • Solvents.
Adsorption Column Chromatography:-- Microscale Columns • Packing the Column • Sample Preparation • The Macroscale Technique • Elution • Bands • Experimental Conditions • Project Ideas • Other Resources • Sorbent Selection Table.
Gel filtration:-- Example Project
Other Systems of Chromatography:-- Electrophoresis • Other Methods
Suggested Projects:-- Variables for Further Study • A Final Note on All Techniques
References
Methods and topics discussed:
Paper Chromatography:-- A Simple Experiment Tells All • Chromatography • How the Method of Paper Chromatography Works • Solvents • The Spot Technique of Paper Chromatography • Materials Needed • Matching Controls • Reagents • Preparation of Unidentified Mixtures Prior to Separation • Technique of Paper Chromatography • Practice Runs with Food Dyes • Testing Unknowns • Some Hazardous Solvents to Avoid • Selecting a Solvent • Some Visualization Reagents • Quantitative Analysis.
Thin Layer Chromatography (TLC):-- How to Coat Glass Microscope Slides • TLC Kits • Solvents.
Adsorption Column Chromatography:-- Microscale Columns • Packing the Column • Sample Preparation • The Macroscale Technique • Elution • Bands • Experimental Conditions • Project Ideas • Other Resources • Sorbent Selection Table.
Gel filtration:-- Example Project
Other Systems of Chromatography:-- Electrophoresis • Other Methods
Suggested Projects:-- Variables for Further Study • A Final Note on All Techniques
References
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