The Low Molecular Weight Proteome: Methods and Protocols

Small proteins with molecular weights of <25 kDa are involved in major biological processes such as ribosome formation, stress adaption and cell cycle control. The study of the low-molecular-weight proteome has identified many central regulators of biology such as cytokines, chemokines, peptide hormones and proteolytic fragments of larger proteins. Due to the unique features of these proteins, the technical challenges are different from those in ”common” proteomics. InThe Low Molecular Weight Proteome: Methods and Proolsexpert researchers from the fieldprovide prools for analysis of low molecular weight proteins and peptides, prools for such methods applied in clinical research and an up-to-date review ofquantitative protein profiling by labeling. These includemethods suitable for both peptide and protein analysis with focus on methods and application that can be used for small protein analysis.Written in the highly successfulMethods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,The Low Molecular Weight Proteome: Methods and Proolsis auseful resource for experienced proteomics practitioners as well as an aid to newcomers who wish to become acquainted with the theory and practice of a wide array of methods in analyzing small proteins or peptides.