In-vitro Evaluation of Antagonistic Potentials of Some Yeast Isolates Against Different Plant Pathogenic Fungi

Research Paper (postgraduate) from the year 2010 in the subject Biology - Botany, , language: English, abstract: Research on the control of plant diseases by phyllosphere applied biological control agents has produced a wealth of informations on a wide range of fungal and bacterial biocontrol agents and their applications for controlling diseases affecting leaves, flowers and fruits. Research into their mode of action and ecological adaptation has provided critical insights that have increased commercial utilization of phyllosphere applied biocontrol agents has proven to be challangeing because the leaf surface presents a relatively hostile environment for introduced microbes with a relative paucity of available nutrients, wide water availability fluxes, direct exoposure to ultraviolet radiation and infrared radiation and competition with other phyllosphere colonist.

Foliar applied biocontrol agents do not have the avoidance strategy of endophytic growth available to pathogens and therefore must primarily utilize a tolerance strategy whereby they colonise and survive on the leaf surface or in protected sites that may or may not be the same as the pathogen they are intended to control. Because of the variable effects of the biological and physical environment, the vast majority of phyllosphere applied biocontrol agents research has been focused on glasshouse or storage environment wherethe physical environment is more predictable and stable.

Many of the early studies aimed at the study of the mode of action and evaluation of the efficacy of some potential biocontrol bacteria, such as Brevibaccilus subtilis, producer of antibiotics, however, the application of such bacteria on fruit did not prove to be commercially acceptable. Wilson and Wisniewski indicated the following characteristics of an ideal antagonist: genetic stability, efficacy at low concentrations and against a wide range of pathogens on various fruit products, simple nutritional requirements, surviva